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Alpha Innotech alphalmagertm 2000 imaging system
Alphalmagertm 2000 Imaging System, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alphalmagertm+2000+imaging+system/alphalmagertm+2000+imaging+system/pm38842666-53-27-31
Average 90 stars, based on 1 article reviews
alphalmagertm 2000 imaging system - by Bioz Stars, 2026-10
90/100 stars

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Imaging:

Article Title: Sinomenine improves renal fibrosis by regulating mesenchymal stem cell-derived exosomes and affecting autophagy levels.
Article Snippet: Funding information The fourth round of Key Disciplines Construction Plan of Municipal and County Co-construction of TCM in Ningbo Integrated Traditional Chinese and Western Medicine Nephropathy Science, Grant/Award Number: 2022-ZX04; Yuyao City Health Science and Technology Plan Project, Grant/Award Number: 2020YYB07 Abstract Background: This study attempts to investigate the therapeutic effect of sinomenine on renal fibrosis and its mechanism.. Methods: The 8-week-old C57BL/6 male mice were randomly divided into sham group, UUO model group, UUO sinomenine group (UUO + Sino 50), UUO + sinomenine group (UUO + Sino 100), UUO + exosome group (exo), and UUO + exo-inhibitor.. The pathological changes of kidney were observed by H&E staining, the degree of renal interstitial fibrosis was detected by MASSON and Sirius red staining, and the expressions of fibrosis and autophagy markers were detected by realtime fluorescence quantitative PCR and WB.

Article Title: MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1
Article Snippet: Protein bands were visualized using ECL Kits (ml095429, Mlbio, Shanghai, China). .. Density of protein bands was examined with AlphalmagerTM 2000 Imaging System (Alpha Innotech, USA). .. MLE-12 cells were fixed with 4 % paraformaldehyde (PN4204, G-CLONE) and exposed to BSA (ab245930, Abcam) for 25 min. Then, cells were incubated with antibodies against α-SMA (ab124964, 1/300, Abcam) overnight and FITC-labelled secondary antibody (ab7086, 1/2000, Abcam) for 1 h. Nuclei were dyed with DAPI (ab285390, Abcam), and images were obtained using a microscope (Olympus, Japan). α-SMA expression was quantified using Image J software (National Institutes of Health, Maryland, USA).

Article Title: Exosome-derived miR-142-5p from liver stem cells improves the progression of liver fibrosis by regulating macrophage polarization through CTSB.
Article Snippet: .. Finally, the AlphalmagerTM 2000 imaging system (Alpha Innotech, USA) was used to analyze the gray value of the protein band, and the expression of β-actin was used as an internal reference. ..

Article Title: CircEXOC5 facilitates cell pyroptosis via epigenetic suppression of Nrf2 in septic acute lung injury.
Article Snippet: Acute lung injury (ALI) caused by sepsis is characterized by a destructive high inflammatory response in lungs, which is the ultimate cause of high mortality to patients diagnosed with sepsis.. The objective of the present study is to explore the effect and related mechanisms of circEXOC5 on pyroptosis in septic ALI.. Sepsis ALI mouse model was induced and established by CLP induction and sepsis MPVEC cell model by LPS.

Article Title: Mir22hg facilitates ferritinophagy-mediated ferroptosis in sepsis by recruiting the m6A reader YTHDC1 and enhancing Angptl4 mRNA stability.
Article Snippet: Following blocking, PVDF membranes were incubated for 12 h at 4 °C with the indicated primary antibodies (Abcam, Cambridge, UK): GPX4 (1:1000, #ab125066), SLC3A2 (1:1000, # ab303510), LC3II/I (0.5 μg/mL, #ab62721), p62 (1:1000, # ab109012), FTH1 (1:1000, #ab183781), NCOA4 (1:1000, #ab314553), YTHDC1 (1:1000, # ab259990), Angptl4 (1:1000, #ab196746) and GAPDH (1:10000, #ab181602). .. An incubation of the PVDF membrane with goat anti-rabbit secondary antibody (1:2000, #ab6721) was then performed for 50 min. Density of protein bands was quantified using the AlphalmagerTM 2000 Imaging System (Alpha Innotech, San Leandro, USA). .. Distribution of ferrous iron in lysosomes After incubation of MLE-12 cells in 96-well plates, the medium was removed, followed by the addition of 50 nM LysoTracker Green (Beyotime) and 1 mM FerroOrange (Dojindo, Japan).

Article Title: CREB3L4 promotes angiogenesis and tumor progression in gastric cancer through regulating VEGFA expression.
Article Snippet: The membranes were stained with anti-CREB3L4 (1:1000 dilution, Santa Cruz Biotechnology, cat no. 390850), antiVEGFA (10 μg/mL, Abcam, cat no. 1316), and anti-β-actin (1:2000 dilution, Abcam, cat no. 32572) primary antibodies followed by 1-h incubation with a goat anti-rabbit secondary antibody (1:2000 dilution, Abcam, cat no. 6721). .. Then the protein bands were visualized and quantified using the AlphalmagerTM 2000 Imaging System (Alpha Innotech, San Leandro, USA). .. All data were analyzed using SPSS (version 24.0) and presented as mean ± standard deviation from at least three independent measurements.

Article Title: Anthocyanins improve liver fibrosis in mice by regulating the autophagic flux level of hepatic stellate cells by mmu_circ_0000623
Article Snippet: After incubating overnight at 4°C with the designated primary antibody, the PVDF membrane was washed with tri- buffered saline (TBS) (Sigma- Aldrich) and then incubated with secondary antibody (diluted 1:2000) for 1 hour at room temperature. .. Finally, the AlphalmagerTM 2000 imaging system (Alpha Innotech) was used to analyze the gray value of the protein band, and the expression of β- actin was used as an internal reference. .. 2.8 | RNA isolation and real- time quantitative PCR (qRT- PCR) Trizol reagent (ThermoFisher) was used to isolate total RNA from liver tissues or mHSCs and perform reverse transcription.

Article Title: Autophagy affects hepatic fibrosis progression by regulating macrophage polarization and exosome secretion.
Article Snippet: .. Finally, the AlphalmagerTM 2000 imaging system (Alpha Innotech, USA) was used to analyze the gray value of the protein band, and the expression of β-actin was used as an internal reference. ..

Expressing:

Article Title: Sinomenine improves renal fibrosis by regulating mesenchymal stem cell-derived exosomes and affecting autophagy levels.
Article Snippet: Funding information The fourth round of Key Disciplines Construction Plan of Municipal and County Co-construction of TCM in Ningbo Integrated Traditional Chinese and Western Medicine Nephropathy Science, Grant/Award Number: 2022-ZX04; Yuyao City Health Science and Technology Plan Project, Grant/Award Number: 2020YYB07 Abstract Background: This study attempts to investigate the therapeutic effect of sinomenine on renal fibrosis and its mechanism.. Methods: The 8-week-old C57BL/6 male mice were randomly divided into sham group, UUO model group, UUO sinomenine group (UUO + Sino 50), UUO + sinomenine group (UUO + Sino 100), UUO + exosome group (exo), and UUO + exo-inhibitor.. The pathological changes of kidney were observed by H&E staining, the degree of renal interstitial fibrosis was detected by MASSON and Sirius red staining, and the expressions of fibrosis and autophagy markers were detected by realtime fluorescence quantitative PCR and WB.

Article Title: Exosome-derived miR-142-5p from liver stem cells improves the progression of liver fibrosis by regulating macrophage polarization through CTSB.
Article Snippet: .. Finally, the AlphalmagerTM 2000 imaging system (Alpha Innotech, USA) was used to analyze the gray value of the protein band, and the expression of β-actin was used as an internal reference. ..

Article Title: Anthocyanins improve liver fibrosis in mice by regulating the autophagic flux level of hepatic stellate cells by mmu_circ_0000623
Article Snippet: After incubating overnight at 4°C with the designated primary antibody, the PVDF membrane was washed with tri- buffered saline (TBS) (Sigma- Aldrich) and then incubated with secondary antibody (diluted 1:2000) for 1 hour at room temperature. .. Finally, the AlphalmagerTM 2000 imaging system (Alpha Innotech) was used to analyze the gray value of the protein band, and the expression of β- actin was used as an internal reference. .. 2.8 | RNA isolation and real- time quantitative PCR (qRT- PCR) Trizol reagent (ThermoFisher) was used to isolate total RNA from liver tissues or mHSCs and perform reverse transcription.

Article Title: Autophagy affects hepatic fibrosis progression by regulating macrophage polarization and exosome secretion.
Article Snippet: .. Finally, the AlphalmagerTM 2000 imaging system (Alpha Innotech, USA) was used to analyze the gray value of the protein band, and the expression of β-actin was used as an internal reference. ..

Membrane:

Article Title: CircEXOC5 facilitates cell pyroptosis via epigenetic suppression of Nrf2 in septic acute lung injury.
Article Snippet: Acute lung injury (ALI) caused by sepsis is characterized by a destructive high inflammatory response in lungs, which is the ultimate cause of high mortality to patients diagnosed with sepsis.. The objective of the present study is to explore the effect and related mechanisms of circEXOC5 on pyroptosis in septic ALI.. Sepsis ALI mouse model was induced and established by CLP induction and sepsis MPVEC cell model by LPS.

Article Title: Mir22hg facilitates ferritinophagy-mediated ferroptosis in sepsis by recruiting the m6A reader YTHDC1 and enhancing Angptl4 mRNA stability.
Article Snippet: Following blocking, PVDF membranes were incubated for 12 h at 4 °C with the indicated primary antibodies (Abcam, Cambridge, UK): GPX4 (1:1000, #ab125066), SLC3A2 (1:1000, # ab303510), LC3II/I (0.5 μg/mL, #ab62721), p62 (1:1000, # ab109012), FTH1 (1:1000, #ab183781), NCOA4 (1:1000, #ab314553), YTHDC1 (1:1000, # ab259990), Angptl4 (1:1000, #ab196746) and GAPDH (1:10000, #ab181602). .. An incubation of the PVDF membrane with goat anti-rabbit secondary antibody (1:2000, #ab6721) was then performed for 50 min. Density of protein bands was quantified using the AlphalmagerTM 2000 Imaging System (Alpha Innotech, San Leandro, USA). .. Distribution of ferrous iron in lysosomes After incubation of MLE-12 cells in 96-well plates, the medium was removed, followed by the addition of 50 nM LysoTracker Green (Beyotime) and 1 mM FerroOrange (Dojindo, Japan).

Incubation:

Article Title: CircEXOC5 facilitates cell pyroptosis via epigenetic suppression of Nrf2 in septic acute lung injury.
Article Snippet: Acute lung injury (ALI) caused by sepsis is characterized by a destructive high inflammatory response in lungs, which is the ultimate cause of high mortality to patients diagnosed with sepsis.. The objective of the present study is to explore the effect and related mechanisms of circEXOC5 on pyroptosis in septic ALI.. Sepsis ALI mouse model was induced and established by CLP induction and sepsis MPVEC cell model by LPS.

Article Title: Mir22hg facilitates ferritinophagy-mediated ferroptosis in sepsis by recruiting the m6A reader YTHDC1 and enhancing Angptl4 mRNA stability.
Article Snippet: Following blocking, PVDF membranes were incubated for 12 h at 4 °C with the indicated primary antibodies (Abcam, Cambridge, UK): GPX4 (1:1000, #ab125066), SLC3A2 (1:1000, # ab303510), LC3II/I (0.5 μg/mL, #ab62721), p62 (1:1000, # ab109012), FTH1 (1:1000, #ab183781), NCOA4 (1:1000, #ab314553), YTHDC1 (1:1000, # ab259990), Angptl4 (1:1000, #ab196746) and GAPDH (1:10000, #ab181602). .. An incubation of the PVDF membrane with goat anti-rabbit secondary antibody (1:2000, #ab6721) was then performed for 50 min. Density of protein bands was quantified using the AlphalmagerTM 2000 Imaging System (Alpha Innotech, San Leandro, USA). .. Distribution of ferrous iron in lysosomes After incubation of MLE-12 cells in 96-well plates, the medium was removed, followed by the addition of 50 nM LysoTracker Green (Beyotime) and 1 mM FerroOrange (Dojindo, Japan).



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Alpha Innotech alphalmagertm 2000 imaging system
Alphalmagertm 2000 Imaging System, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alphalmagertm+2000+imaging+system/alphalmagertm+2000+imaging+system/pm38842666-53-27-31
Average 90 stars, based on 1 article reviews
alphalmagertm 2000 imaging system - by Bioz Stars, 2026-10
90/100 stars
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